Difference between revisions of "Part:BBa K2213001:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: | To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: | ||
- concentration of Tetracyclin inducer (induces EutMN synthesis) | - concentration of Tetracyclin inducer (induces EutMN synthesis) | ||
Revision as of 20:23, 29 October 2017
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how you used this part and how it worked out.
Manchester 2017 - BBa_K2213001
To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: - concentration of Tetracyclin inducer (induces EutMN synthesis) - concentration of IPTG inducer (induces EutS synthesis) - Harvest time (time after induction) - Temperature - Growth Medium (LB and TB) / from this 22 flask investigation we were able to make surface plots to visulise our findings. /
- 2 TEMPERATURE DoE SURFACE PLOTS***
/ Figure1:
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