Difference between revisions of "Part:BBa K2232020"

Revision as of 19:04, 29 October 2017

NOTOC__ P43_TSLV1-CA-alpha_rrnBT1

This part is expressed in B.subsilis to increase the germinability of the spore.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 851
Illegal NotI site found at 1138
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 831
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 376
Illegal SapI.rc site found at 534
Illegal SapI.rc site found at 846

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+NOTOC__
+<partinfo>BBa_K2232014 short</partinfo>
+This part is expressed in B.subsilis to increase the germinability of the spore.
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+The gerA operon from Bacillus subtilis encodes the spore’s germinant receptor for L-alanine. Overexpression of the tricistronic gerA operon will increase markedly the rates of germination of Bacillus subtilis spores with L-alanine,in particular at low L-alanine concentrations.
+The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous gerAa promoter. It is also active during germination.
+This device consists mainly of the sspB promoter followed by the first 303bp of the gerA operon (first 303bp of the gerAa gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the gerAa region in the BioBrick and the endogenous gerA sequence inserts the sspB promoter upstream of the spoVA operon,which promote the germination of Bacillus subtilis.
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K2232014 SequenceAndFeatures</partinfo>
+<!-- Uncomment this to enable Functional Parameter display
+===Functional Parameters===
+<partinfo>BBa_K2232014 parameters</partinfo>
+<!-- -->