Difference between revisions of "Part:BBa K2253002"

Revision as of 18:54, 29 October 2017

Optimized Blue Chromoprotein

As previously stated, this is the original blue chromoprotein sequence (BBa_K592009) that has been codon optimized. The part has been made a composite part with the promotor and RBS sequence, BBa_K608002 in the pSB1C3 BioBrick backbone. Codon optimization allows it to function more efficiently in E.coli, therefore creating a lower metabolic burden on the cells that it is encoded in. When this sequence is put into a vector and transformed into a plasmid it shows a blue phenotype. Codon optimization also makes this part more stable, as the color is expected to be maintained for a longer period of time than the original, making it a good candidate to use as a biosensor for things like toxins, such as lead.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 694
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2253002 short</partinfo>
-As previously stated, this is the original blue chromoprotein sequence (BBa_K592009) that has been codon optimized. The part has been made a composite part with the promotor and RBS sequence, BBa_K608002 in the pSB1C3 biobrick backbone. Codon optimization allows it to function more efficiently in E.coli, therefore creating a lower metabolic burden on the cells that it is encoded in. When these sequence is put into a vector and transformed into a plasmid it shows a blue phenotype. Codon optimization also makes this part more stable than the original, making It a good candidate to use as a biosensor for things like toxins such as lead.
+As previously stated, this is the original blue chromoprotein sequence (BBa_K592009) that has been codon optimized. The part has been made a composite part with the promotor and RBS sequence, BBa_K608002 in the pSB1C3 BioBrick backbone. Codon optimization allows it to function more efficiently in E.coli, therefore creating a lower metabolic burden on the cells that it is encoded in. When this sequence is put into a vector and transformed into a plasmid it shows a blue phenotype. Codon optimization also makes this part more stable, as the color is expected to be maintained for a longer period of time than the original, making it a good candidate to use as a biosensor for things like toxins, such as lead.
 <!-- Add more about the biology of this part here