Difference between revisions of "Part:BBa K2271060:Experience"

Revision as of 18:49, 29 October 2017

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Please enter how you used this part and how it worked out.

Applications of BBa_K2271060

For testing this part we used a fusion with the N-Terminus of a Peroxisomal Membrane anchor. We co-expressed this construct with Gus-PTS to perform a GUS Assay.(oder: We performed a GUS-Assay by targetting GUS(beta-Glucuronidase) to the Peroxisome using a GUS-PTS1.) The secreted GUS in the supernatant was measured with the turnover of 4-methylumbelliferyl-beta-D-glucuronide to 4-methyl umbelliferone (4-MUG). The fluorescent 4-MUG was measured with a plate reader (excitation: 365 nm, emission: 465 nm). Different peroxisomal membrane anchors were tested using the GUS-Assay. The highest activity of GUS could be measured in the supernatant of Pex15 (Link to part) as a Membrane Anchor.

User Reviews

UNIQbb1f94e5e7c0e64d-partinfo-00000000-QINU UNIQbb1f94e5e7c0e64d-partinfo-00000001-QINU

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 ===Applications of BBa_K2271060===
+For testing this part we used a fusion with the N-Terminus of a Peroxisomal Membrane anchor. We co-expressed this construct with Gus-PTS to perform a GUS Assay.(oder: We performed a GUS-Assay by targetting GUS(beta-Glucuronidase) to the Peroxisome using a GUS-PTS1.) The secreted GUS in the supernatant was measured with the turnover of 4-methylumbelliferyl-beta-D-glucuronide to 4-methyl umbelliferone (4-MUG). The fluorescent 4-MUG was measured with a plate reader (excitation: 365 nm, emission: 465 nm).
+Different peroxisomal membrane anchors were tested using the GUS-Assay. The highest activity of GUS could be measured in the supernatant of Pex15 (Link to part) as a Membrane Anchor.
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K2271060 StartReviews</partinfo>