Difference between revisions of "Part:BBa K2333407"

Protein degradation tag A is the strongest of the 6 protein degradation tags that William and Mary 2017 characterized, and is associated with the E. Coli orthogonal protease mf-Lon (<partinfo>Bba_K2333011</partinfo>). Therefore this part would have the greatest degradation rate of the 6 protein degradation tags and it would reach steady-state fluorescence values the quickest. This part contains J23100 constitutive promoter, <partinfo>Bba_B0034 (RBS),pdt A, a double stop codon and <partinfo>Bba_B0015</partinfo> (double terminator) in the William and Mary iGEM Universal Nucleotide Sequences (UNS) format. The sfGFP reporters have been codon-optimized for E. coli and feature a double stop codon for enhanced efficiency. In order to demonstrate that protein degradation tags operated similarily regardless of the tagged protein, sfGFP reporters that were analogous to the mScarlet-I parts <partinfo>Bba_K2333413<partinfo> to <partinfo>Bba_K2333419<partinfo> were built and characterized. This demonstrates that the protein degradation tags are modular and that they have differential strengths even when they are tagged on different proteins.