Difference between revisions of "Part:BBa K2333413"
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<partinfo>BBa_K2333413 short</partinfo> | <partinfo>BBa_K2333413 short</partinfo> | ||
| − | mScarlet | + | This part is contained in a suite of protein degradation tagged mScarlet reporters under the control of the strong constitutive promoter J23100. These parts, in combination with inducible mf-Lon protease constructs, allowed us to characterize the degradation properties of each protein degradation tag (pdt) on a plasmid-based system. We successfully demonstrated distinct levels of protein degradation by each of the 6 pdt’s, and mScarlet reporters have been codon-optomized for E. coli and feature a double stop codon for enhanced efficiency. This specific part is a tagless control construct (J23100 mScarlet with no pdt) which can be used as a comparison for protein degradation with pdt's. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | mScarlet-I with no pdt under the control of the constitutive promoter J23100 | ||
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Revision as of 17:25, 29 October 2017
UNS J23100 mScarlet-I
This part is contained in a suite of protein degradation tagged mScarlet reporters under the control of the strong constitutive promoter J23100. These parts, in combination with inducible mf-Lon protease constructs, allowed us to characterize the degradation properties of each protein degradation tag (pdt) on a plasmid-based system. We successfully demonstrated distinct levels of protein degradation by each of the 6 pdt’s, and mScarlet reporters have been codon-optomized for E. coli and feature a double stop codon for enhanced efficiency. This specific part is a tagless control construct (J23100 mScarlet with no pdt) which can be used as a comparison for protein degradation with pdt's.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70
Illegal NotI site found at 605 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]