Difference between revisions of "Part:BBa K2207024"

Revision as of 16:14, 29 October 2017

Modified T7 promoter 1

BBa_K2207024

Modified Promoter T7

This part is a promoter derived from wild-type T7 promoter with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.

Usage and Biology

We put the Biobrick E1010(mRFP) behind the K2207024 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207024 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.

According to the data, it is easy to find that the ratio of relative strength of K2207024 and K525998 is 0.26, indicating that this part may show lower expression efficiency relative to the WT-T7 promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 14: / Line 14: @@
 We put the Biobrick E1010(mRFP) behind the K2207024 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207024 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3).
 And the result is shown in the figure below.
 https://static.igem.org/mediawiki/2017/4/4b/ZJU_China_R_24.png
 According to the data, it is easy to find that the ratio of relative strength of K2207024 and K525998 is 0.26, indicating that this part may show lower expression efficiency relative to the WT-T7 promoter.