Difference between revisions of "Part:BBa K2207024"
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control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene. | control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene. | ||
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We put the Biobrick E1010(mRFP) behind the K2207024 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207024 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). | We put the Biobrick E1010(mRFP) behind the K2207024 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207024 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). | ||
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According to the data, it is easy to find that the ratio of relative strength of K2207024 and K525998 is 0.26, indicating that this part may show lower expression efficiency relative to the WT-T7 promoter. | According to the data, it is easy to find that the ratio of relative strength of K2207024 and K525998 is 0.26, indicating that this part may show lower expression efficiency relative to the WT-T7 promoter. | ||
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Revision as of 14:53, 29 October 2017
Modified T7 promoter 1
BBa_K2207024
Modified Promoter T7
This part is a promoter derived from wild-type T7 promoter with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]