Difference between revisions of "Part:BBa K2315046"
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a generator of AHL molecules and a reporter which can receive molecules and activate the downstream genes’ expression. However, there is rarely molecule
 
a generator of AHL molecules and a reporter which can receive molecules and activate the downstream genes’ expression. However, there is rarely molecule
 
converter, which can receive a kind of AHL molecule and convert it into another AHL molecule. In our project this year, we pay attention to two kinds of
 
converter, which can receive a kind of AHL molecule and convert it into another AHL molecule. In our project this year, we pay attention to two kinds of
−
QS systems: Las and Rpa. We successfully construct a <c>‘converter’– it can receive Rpa molecules and then generate Las molecules.</b> This device
+
QS systems: Las and Rpa. We successfully construct a <b>‘converter’– it can receive Rpa molecules and then generate Las molecules.</b> This device
 
achieves a simple logic circuit, moreover, It may lay the foundation for constructing a much more complex logic circuit.
 
achieves a simple logic circuit, moreover, It may lay the foundation for constructing a much more complex logic circuit.
 
 

Revision as of 14:38, 29 October 2017

Rpa-Las converter

Group: Shanghaitech iGEM 2017

In synthetic biology, quorum sensing system (QS system) has been researched as a way of bacteria communication. A whole system always concludes two parts: a generator of AHL molecules and a reporter which can receive molecules and activate the downstream genes’ expression. However, there is rarely molecule converter, which can receive a kind of AHL molecule and convert it into another AHL molecule. In our project this year, we pay attention to two kinds of QS systems: Las and Rpa. We successfully construct a ‘converter’– it can receive Rpa molecules and then generate Las molecules. This device achieves a simple logic circuit, moreover, It may lay the foundation for constructing a much more complex logic circuit.

Converter figure 0.png
Converter figure 1.png
Converter figure 3.png
Converter figure 4.png
Converter figure 5.png
Converter figure 6.png
Converter figure 7.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 85
    Illegal NheI site found at 108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 571
    Illegal XhoI site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
    Illegal NgoMIV site found at 278
    Illegal NgoMIV site found at 563
    Illegal NgoMIV site found at 651
    Illegal AgeI site found at 1335
  • 1000
    COMPATIBLE WITH RFC[1000]