Difference between revisions of "Part:BBa K2243005:Design"
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− | ===Design | + | ===Usage=== |
− | + | C12_Bxb1 gp35 is one of our best expression system. We added RBS C12 upstream the Bxb1 gp35 coding sequence (BBa_K22430012). | |
+ | |||
+ | ===Biology=== | ||
+ | The Bxb1 coding sequence is the part BBa_K22430012. | ||
+ | |||
+ | ===Design note=== | ||
+ | We construct this structure by Golden Gate Assembly. The C12 RBS is generate by random mutation. | ||
+ | |||
+ | ===Characterize=== | ||
+ | We characterize the C12_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006). | ||
+ | |||
Latest revision as of 14:35, 29 October 2017
C12_Bxb1 gp35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 212
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 486
Illegal XhoI site found at 573 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1125
Illegal NgoMIV site found at 1212
Illegal AgeI site found at 262 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1320
Usage
C12_Bxb1 gp35 is one of our best expression system. We added RBS C12 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
Biology
The Bxb1 coding sequence is the part BBa_K22430012.
Design note
We construct this structure by Golden Gate Assembly. The C12 RBS is generate by random mutation.
Characterize
We characterize the C12_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006).
Source
Mycobacterium Phage Bxb1