Difference between revisions of "Part:BBa K2243003:Design"
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<partinfo>BBa_K2243003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2243003 SequenceAndFeatures</partinfo> | ||
+ | ===Usage=== | ||
+ | D7_Bxb1 gp35 is one of our best expression system. We added RBS D7 upstream the Bxb1 gp35 coding sequence (BBa_K22430012). | ||
− | === | + | ===Biology=== |
− | + | The Bxb1 coding sequence is the part BBa_K22430012. | |
+ | ===Design Notes=== | ||
+ | We construct this structure by Gibson Assembly. The D7 RBS is generate by random mutation. | ||
+ | ===Characterize=== | ||
+ | We characterize the D7_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006). | ||
===Source=== | ===Source=== |
Latest revision as of 14:32, 29 October 2017
D7_Bxb1 gp35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 466
Illegal XhoI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1105
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1300
Usage
D7_Bxb1 gp35 is one of our best expression system. We added RBS D7 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
Biology
The Bxb1 coding sequence is the part BBa_K22430012.
Design Notes
We construct this structure by Gibson Assembly. The D7 RBS is generate by random mutation.
Characterize
We characterize the D7_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006).
Source
Mycobacterium Phage Bxb1