Difference between revisions of "Part:BBa K2243002:Design"
Woodsorrel (Talk | contribs) |
|||
Line 5: | Line 5: | ||
<partinfo>BBa_K2243002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2243002 SequenceAndFeatures</partinfo> | ||
+ | ===Usage=== | ||
+ | D4_Bxb1 gp35 is one of the best expression system. We added RBS D4 upstream the Bxb1 gp35 coding sequence (BBa_K22430012). | ||
− | === | + | ===Biology=== |
− | + | The Bxb1 coding sequence is the part BBa_K22430012. | |
+ | ===Design Notes=== | ||
+ | We construct this structure by Gibson Assembly. The D4 RBS is generate by random mutation. | ||
Revision as of 14:23, 29 October 2017
D4_Bxb1 gp35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 466
Illegal XhoI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1105
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1300
Usage
D4_Bxb1 gp35 is one of the best expression system. We added RBS D4 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
Biology
The Bxb1 coding sequence is the part BBa_K22430012.
Design Notes
We construct this structure by Gibson Assembly. The D4 RBS is generate by random mutation.
Source
Mycobacterium Phage Bxb1