Difference between revisions of "Part:BBa K2374003:Design"
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According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.
 
According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.
  
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[[Image:SNR.png|thumb|265px|'''Figure 2''': SNR comparison.]]
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<br><table><tr><th>
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[[Image:Delay.png|thumb|265px|'''Figure 3''': Delay comparison.]]
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[[Image:SNR.png|thumb|265px|'''Figure 2''': SNR comparison.]]</th><th>
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[[Image:Max_intensity.png|thumb|265px|'''Figure 4''': Max intensity conparision.]]
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[[Image:Delay.png|thumb|265px|'''Figure 3''': Delay comparison.]]</th><th>
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[[Image:Max_intensity.png|thumb|265px|'''Figure 4''': Max intensity conparision.]]</th></tr></table>
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https://static.igem.org/mediawiki/2017/c/cc/2017tongji_image_registry_UASTHtest.png
 
https://static.igem.org/mediawiki/2017/c/cc/2017tongji_image_registry_UASTHtest.png

Revision as of 12:30, 29 October 2017


ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
    Illegal XhoI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 535
    Illegal BsaI site found at 1501
    Illegal BsaI.rc site found at 253
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 1168


Design Notes

site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG

According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.


Figure 2: SNR comparison.
Figure 3: Delay comparison.
Figure 4: Max intensity conparision.


2017tongji_image_registry_UASTHtest.png

Source

Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)

References

JANICE A. FISCHER, et al. GAL4 activates transcription in Drosophila. Nature 332, 853 - 856 (1988)