Difference between revisions of "Part:BBa K2382005"
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<partinfo>BBa_K2382005 short</partinfo>
 
<partinfo>BBa_K2382005 short</partinfo>
  
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By ligating these two different parts as a fusion protein, it is supposed to
 
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raise the solubility of our protein, F420-dependent glucose-6- phosphate
 
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dehydrogenase (FGD).
 
  
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===Usage and Biology===
 
  
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===<span class='h3bb'>Sequence and Features</span>===
 
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K2382005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2382005 SequenceAndFeatures</partinfo>
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===Design Notes===
 
  
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This is a fusion protein made out of Thioredoxin and FGD.
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===Usage and Biology===
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Both of them have different function.
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By ligating these two different parts as a fusion protein, it is supposed to
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Thioredoxin can help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.
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raise the solubility of our protein, F420-dependent glucose-6- phosphate
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FGD could help the electron transferred from G6P(Glucose 6-phosphate) to the F420(It is a flavin derivative, and it plays like a coenzyme that could help the redox reactions in methanogens ,many Actinobacteria, especially in bacterial lineages.)
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dehydrogenase (FGD).
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Finally, the F420 would be reduced to F420H2,attaching two hydrogens and electron on it.
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This fusion protein will help us to convert electrons to reduce the compound via MSMEG5998.
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===Characterization of the Thioredoxin with polylinker===
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===References===

Revision as of 12:17, 29 October 2017

Thioredoxin-FGD fusion protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1117
    Illegal BamHI site found at 405
    Illegal XhoI site found at 411
    Illegal XhoI site found at 1383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal NgoMIV site found at 975
    Illegal AgeI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

By ligating these two different parts as a fusion protein, it is supposed to raise the solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).


Characterization of the Thioredoxin with polylinker

References