Difference between revisions of "Part:BBa K2203001"
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<partinfo>BBa_K2203001 short</partinfo> | <partinfo>BBa_K2203001 short</partinfo> | ||
| − | A composition of | + | A composition of the coding sequence for the protein GamS with a T7 promoter and terminator as |
well as a 6X His-tag for easy transcription and purification of the GamS protein. GamS is a protein | well as a 6X His-tag for easy transcription and purification of the GamS protein. GamS is a protein | ||
that protects linear DNA templates from degradation in a cell-free environment. It is used to enhance | that protects linear DNA templates from degradation in a cell-free environment. It is used to enhance | ||
Revision as of 12:07, 29 October 2017
T7-GamS-HisTag
A composition of the coding sequence for the protein GamS with a T7 promoter and terminator as well as a 6X His-tag for easy transcription and purification of the GamS protein. GamS is a protein that protects linear DNA templates from degradation in a cell-free environment. It is used to enhance cell-free protein synthesis by inhibiting exonuclease activity. For a detailed description and experimental demonstration of the effect of GamS on cell-free protein synthesis, please refer to the page of GamS (BBa_K2203000). This composite part includes a T7 promoter and terminator for enhanced transcription of GamS-6XHis during growth of bacteria transformed with the plasmid containing this part. GamS can then be specifically purified or detected in the cell lysate by making use of the 6XHis tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 336
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 323
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]