Difference between revisions of "Part:BBa K2325001"
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===Enzymatic properities===
 
===Enzymatic properities===
 
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<b>Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH</b>
 
<b>Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH</b>
 
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<b>Figure 2.The Michaelis constant of phosphite dehydrogenase</b>
 
<b>Figure 2.The Michaelis constant of phosphite dehydrogenase</b>
 
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[[File:BBa K2325001--5.png]]<br />
 
[[File:BBa K2325001--5.png]]<br />
 
<b>Figure 3.Influence of metal ion on the enzymatic activity of phosphite dehydrogenase</b>
 
<b>Figure 3.Influence of metal ion on the enzymatic activity of phosphite dehydrogenase</b>

Revision as of 11:57, 29 October 2017


Phosphite dehydrogenase (PTDH)

The BBa_K2325001 harbors a coding sequence of Phosphite dehydrogenase (PTDH), which catalyzes the conversion of phosphite to phosphate.

Usage and Biology

Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli.

KH2PO3 + H2O + NAD+ ⇒ KH2PO4 + NADH

Gel analysis

BBa K2325001--1.png

Enzymatic properities

BBa K2325001--2.png
Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH


BBa K2325001--3.png
Figure 2.The Michaelis constant of phosphite dehydrogenase


BBa K2325001--4.png
BBa K2325001--5.png
Figure 3.Influence of metal ion on the enzymatic activity of phosphite dehydrogenase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]