Difference between revisions of "Part:BBa K2325000"
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[[File:BBa_K2325000--3.png|750px]]<br />
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<b>Figure 2.The Michaelis constant of formamidase</b>
 
<b>Figure 2.The Michaelis constant of formamidase</b>
 
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Revision as of 11:53, 29 October 2017


Formamidase (FOR)

The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.

Usage and Biology

Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.

HCO - NH2 + H2O ⇒ HCOOH + NH3

Gel analysis

BBa K2325000--1.png

Enzymatic properties

BBa K2325000--2.png
Figure 1.Enzymatic activity of formamidase under different temperatures and pH


BBa K2325000--3.png
Figure 2.The Michaelis constant of formamidase


BBa K2325000--4.png
BBa K2325000--5.png
Figure 3.Influence of metal ion on the enzymatic activity of formamidase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]