Difference between revisions of "Part:BBa K2305010"
WANGWENJIA (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
It's a GFP/RFP dual fluorescent system | It's a GFP/RFP dual fluorescent system | ||
==Agarose gel electrophoresis== | ==Agarose gel electrophoresis== | ||
| − | We calculated the new part’s base number to be | + | We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. |
[[Image:BIT Figure DNA sequencing K2305010.png|200px|left|K230500]]<br clear="all"> | [[Image:BIT Figure DNA sequencing K2305010.png|200px|left|K230500]]<br clear="all"> | ||
Latest revision as of 11:47, 29 October 2017
GFP/RFP dual fluorescent system with LacI and plac
It's a GFP/RFP dual fluorescent system
Agarose gel electrophoresis
We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing[edit] The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Characterization map
Fig.The Growth curve of E.coil.
Fig.The GFP curve of E.coil.
Fig.The RFP curve of E.coil.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1165
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2846
Illegal AgeI site found at 2958 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1844