Difference between revisions of "Part:BBa K2305001"
 
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Pcat promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.
 
Pcat promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.
  
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==Agarose gel electrophoresis==
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==DNA Sequencing==
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We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
+
We calculated the new part’s base number to be 1007bp. The figure1 is the result of DNA Sequencing. We can make a preliminary judgment that the sequences are correct.
  
 
[[Image:BIT Figure DNA sequencing K2305001-a.PNG |200px|left|K230501a]][[Image:BIT Figure DNA sequencing K2305001-b.PNG |200px|left|K230501b]]<br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305001-a.PNG |200px|left|K230501a]][[Image:BIT Figure DNA sequencing K2305001-b.PNG |200px|left|K230501b]]<br clear="all">

Latest revision as of 11:43, 29 October 2017


cycle amplification system and GFP/RFP dual fluorescent system

Pcat promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.

DNA Sequencing

We calculated the new part’s base number to be 1007bp. The figure1 is the result of DNA Sequencing. We can make a preliminary judgment that the sequences are correct.

K230501a
K230501b

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1525
    Illegal BglII site found at 2704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 4497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383