Difference between revisions of "Part:BBa K2203003"
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<partinfo>BBa_K2203003 short</partinfo> | <partinfo>BBa_K2203003 short</partinfo> | ||
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This composite part consists of a T7 promoter (BBa_J64997) in front of the toehold, a lacZalpha (BBa_I732006) downstream of it and a T7 terminator (BBa_K731721) at the end of it. | This composite part consists of a T7 promoter (BBa_J64997) in front of the toehold, a lacZalpha (BBa_I732006) downstream of it and a T7 terminator (BBa_K731721) at the end of it. | ||
A toehold switch works according to this scheme: when the trigger sequence in form of RNA or ssDNA is present in the sample, the toehold will unfold and lacZalpha will be translated. For more information about toeholds, go to 2017.igem.org/Team:EPFL | A toehold switch works according to this scheme: when the trigger sequence in form of RNA or ssDNA is present in the sample, the toehold will unfold and lacZalpha will be translated. For more information about toeholds, go to 2017.igem.org/Team:EPFL | ||
Revision as of 11:24, 29 October 2017
HepHCV_ToeholdA
This composite part consists of a T7 promoter (BBa_J64997) in front of the toehold, a lacZalpha (BBa_I732006) downstream of it and a T7 terminator (BBa_K731721) at the end of it. A toehold switch works according to this scheme: when the trigger sequence in form of RNA or ssDNA is present in the sample, the toehold will unfold and lacZalpha will be translated. For more information about toeholds, go to 2017.igem.org/Team:EPFL
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]