Difference between revisions of "Part:BBa K2382003"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2382003 short</partinfo>
 
<partinfo>BBa_K2382003 short</partinfo>
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This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS.
 
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We tried to improve this promoter sequence at [https://parts.igem.org/Part:BBa_K525998# BBa_K525998] by adding an lac operator and making it RFC 10 compatible.
 
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===Design Notes===
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===Short Description===
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We use the T7 promotor from Part:BBa_K525998(Group: iGEM11_Bielefeld-Germany  (2011-09-13))
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This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS.
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>sequence (32 bp)
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We tried to improve this promoter sequence at [https://parts.igem.org/Part:BBa_K525998# BBa_K525998] by adding an lac operator and making it RFC 10 compatible.  
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taatacgactcactatagggaaagaggagaaa
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We add additional sequence on it. There are two function in our sequence follow the T7 promotor. One is lac operator, the other is RBS binding site.
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Lac operator could be bound with lac repressor. It could stop the transcription to prevent making protein. Genome of E.coli has the lacI gene. It could translate the repressor to inhibit protein produced. That is, we design the lac operator into our sequence to prevent the leak of expressing protein.
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===References===
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RBS binding site could let the ribosome attach on the mRNA sequence to produce the enzyme.
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These sequence is derived from pET-29a(+) sequence. Because the original part has the restriction site”Xba1”in the sequence, and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, the restriction site would not be recognized by restriction enzyme and cut off. That is, it is safe to use to compose the sequence for igem.
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Revision as of 10:50, 29 October 2017

T7 promoter & Lac operator and RBS from PET-29a


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Short Description

This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS. We tried to improve this promoter sequence at BBa_K525998 by adding an lac operator and making it RFC 10 compatible.

References