Difference between revisions of "Part:BBa I732006"
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<img src="https://parts.igem.org/File:171002_CHARACTERIZATION_T7_LACZA_all.png" height="480" width="640">
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<img src="https://static.igem.org/mediawiki/parts/8/88/171002_CHARACTERIZATION_T7_LACZA_all.png" height="480" width="640">
 
<figcaption>Repeats of T7LacZalpha in T7M15 lysate</figcaption>
 
<figcaption>Repeats of T7LacZalpha in T7M15 lysate</figcaption>
 
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Revision as of 10:44, 29 October 2017


lacZ alpha fragment

In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization T7 lacZalpha

Repeats of T7LacZalpha in T7M15 lysate

Improvements

This part was improved with a T7 promoter and terminator : BBa_K2203002 . This part is also used as a reporter in the following composite parts which are toehold switches : BBa_K2203003 , BBa_K2203004 , BBa_K2203005 , BBa_K2203006 .