Difference between revisions of "Part:BBa K2271105:Experience"
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__NOTOC__
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K2271105===
 
===Applications of BBa_K2271105===
  
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<h1>Import of the fluorescent protein mTurquoise</h1>
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===Import of fluorescent proteins into the peroxisomal matrix===
  
 
<h2>PEX5 variant with wildtype PTS1</h2>
 
<h2>PEX5 variant with wildtype PTS1</h2>
  
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https://static.igem.org/mediawiki/2017/b/b0/Artico_15wtp13.png
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[[Image:Artico_15wtp13.png|frame|'''Figure 1:'''PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
  
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<h2>PEX5 variant with PTS1 variants</h2>
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[[Image:Artico_15p3p13.png|frame|'''Figure 2:'''PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1* P3 and the PEX13 membrane anchor fused to mRuby.]]
  
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<h3> PTS1 variant P2 </h3>
 
  
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https://static.igem.org/mediawiki/2017/a/ae/Artico_15p3p13.png
 
  
  

Revision as of 10:05, 29 October 2017

Applications of BBa_K2271105

Import of fluorescent proteins into the peroxisomal matrix

PEX5 variant with wildtype PTS1

Figure 1:PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1* P3 and the PEX13 membrane anchor fused to mRuby.



User Reviews

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