Difference between revisions of "Part:BBa K1033121"

Revision as of 09:50, 29 October 2017

Miraculin

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.

Part improvement: team Tel-Hai 2017

Group: Tel-Hai 2017

Author: Almog Angel

BBa_K2408023

Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for S. cerevisiae. 2. His tag for purification 3. ADH1 terminator for more effective expression.

Sequence and Features