Difference between revisions of "Part:BBa K2483009:Design"
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Because our other parts (BBa_K2483007 and BBa_K2483008) for this project were also under control of the GAL1 promoter (strong, galactose-induced promoter), we needed to do the same here for it to be a control. | Because our other parts (BBa_K2483007 and BBa_K2483008) for this project were also under control of the GAL1 promoter (strong, galactose-induced promoter), we needed to do the same here for it to be a control. | ||
| − | + | We did not check for illegal restricition sites, so the SpeI sites should have to be mutated out. | |
===Source=== | ===Source=== | ||
Latest revision as of 09:36, 29 October 2017
IAAM and IAAH yeast optimized under GAL1 control
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2301
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2301
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2301
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2301
Illegal NgoMIV site found at 1212
Illegal AgeI site found at 916
Illegal AgeI site found at 2086
Illegal AgeI site found at 2377 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3594
Illegal BsaI.rc site found at 913
Design Notes
Because our other parts (BBa_K2483007 and BBa_K2483008) for this project were also under control of the GAL1 promoter (strong, galactose-induced promoter), we needed to do the same here for it to be a control.
We did not check for illegal restricition sites, so the SpeI sites should have to be mutated out.
Source
The CDS for IAAM and IAAH are from the part BBa_K515100 and codon optimized for yeast. GAL1 and CYC1 are taken from the vector we were working with, which was pYES2/CT.