Difference between revisions of "Part:BBa K2446015:Design"
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===Source=== | ===Source=== | ||
| − | + | This part was assembled with two BBa_K2446008 by Biobrick assembly in pSB1C3. | |
===References=== | ===References=== | ||
Revision as of 06:01, 29 October 2017
8_ZF_21-16 binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
Source
This part was assembled with two BBa_K2446008 by Biobrick assembly in pSB1C3.