Difference between revisions of "Part:BBa K2486009"
| Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Wen Fe<sup>2+</sup> | + | Wen Fe<sup>2+</sup> is added to a system where the DtxR protein is present (which can be achieved by the use of our [https://parts.igem.org/Part:BBa_K2486004 dtxR generator]) the protein enters in it's active state and is able to repress gene expression through its cognate operator. |
| + | In our inverter system we have a modified constitutive promoter (BBa_X) that produces TetR in the absence of active DtxR. The produced TetR in turn can repress a second promoter with the tetR operator. In the presence of Fe<sup>2+</sup> the tetR expression would cease and permit reporter production. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 05:44, 29 October 2017
DtxR inverter using TetR
This part inverts the signal produced by the interaction between DtxR protein and the cognate operator.
Usage and Biology
Wen Fe2+ is added to a system where the DtxR protein is present (which can be achieved by the use of our dtxR generator) the protein enters in it's active state and is able to repress gene expression through its cognate operator. In our inverter system we have a modified constitutive promoter (BBa_X) that produces TetR in the absence of active DtxR. The produced TetR in turn can repress a second promoter with the tetR operator. In the presence of Fe2+ the tetR expression would cease and permit reporter production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 695
Illegal NheI site found at 718 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]