Difference between revisions of "Part:BBa K2267011"
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<partinfo>BBa_K2267011 short</partinfo> | <partinfo>BBa_K2267011 short</partinfo> | ||
| − | SpyTag is a protein, which can form irreversible bond with SpyCatcher. Two coding sequences, Escherichia coli RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from Streptococcus pyogenes fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by adding function protein. | + | SpyTag is a protein, which can form irreversible bond with SpyCatcher. Two coding sequences, <i>Escherichia coli</i> RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from <i>Streptococcus pyogenes</i> fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by adding function protein. |
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Latest revision as of 05:19, 29 October 2017
SpyTag
SpyTag is a protein, which can form irreversible bond with SpyCatcher. Two coding sequences, Escherichia coli RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from Streptococcus pyogenes fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by adding function protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]