Difference between revisions of "Part:BBa K2486026"

Latest revision as of 23:54, 28 October 2017

pGadA-MazF kill switch

[not sent]

Kill switch designed to reduce viability of E. coli upon contact with low pH environments in the range of normal rain drops (5-6).

pGadA promoter was characterized by team Dundee 2016 (BBa_K1962013) with GFP protein in different pH ranges. The GPF coding sequence was substituted by MazF, a endoribonuclease well characterized in kill switch approaches (1). This could limit replication of chassis for paratransgenesis in the environment, while allowing it to thrive inside the mosquito midgut.

Chan, Clement TY, et al. "'Deadman'and'Passcode'microbial kill switches for bacterial containment." Nature chemical biology 12.2 (2016): 82-86.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2486026 short</partinfo>
+[not sent]
 Kill switch designed to reduce viability of E. coli upon contact with low pH environments in the range of normal rain drops (5-6).
-pGadA promoter was characterized by team Dundee 2016 ([https://parts.igem.org/Part:BBa_K1962013 BBa_K1962013]) with GFP protein in different pH ranges. The GPF coding sequence was substituted by MazF, a endoribonuclease well characterized in kill switch approaches (1)
+pGadA promoter was characterized by team Dundee 2016 ([https://parts.igem.org/Part:BBa_K1962013 BBa_K1962013]) with GFP protein in different pH ranges. The GPF coding sequence was substituted by MazF, a endoribonuclease well characterized in kill switch approaches (1). This could limit replication of  chassis for paratransgenesis in the environment, while allowing it to thrive inside the mosquito midgut.
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