Difference between revisions of "Part:BBa I742137"

 
 
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Integral membrane-protein FtsK translocates to the septum at bacterial division, clutches DNA and tugs at it so that replicated DNA monomers that are interdigitated may be excised by the XerCD recombinase complex. See Edinburgh 2007 project background for more detail.
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Integral membrane-protein FtsK translocates to the septum at bacterial division, clutches DNA and tugs at it so that replicated DNA monomers that are interdigitated may be excised by the XerCD recombinase complex. Note that this is the native coding sequence from ''Escherichia coli'', which begins with a TTG start codon and includes two PstI sites. See Edinburgh 2007 project background for more detail.
  
 
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Latest revision as of 09:13, 25 October 2007

DNA motor protein FtsK

Integral membrane-protein FtsK translocates to the septum at bacterial division, clutches DNA and tugs at it so that replicated DNA monomers that are interdigitated may be excised by the XerCD recombinase complex. Note that this is the native coding sequence from Escherichia coli, which begins with a TTG start codon and includes two PstI sites. See Edinburgh 2007 project background for more detail.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1166
    Illegal PstI site found at 1229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 729
    Illegal PstI site found at 1166
    Illegal PstI site found at 1229
    Illegal NotI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3795
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1166
    Illegal PstI site found at 1229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1166
    Illegal PstI site found at 1229
    Illegal AgeI site found at 535
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 2232
    Illegal AgeI site found at 2349
    Illegal AgeI site found at 2388
    Illegal AgeI site found at 2977
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1309
    Illegal SapI.rc site found at 3403