Difference between revisions of "Part:BBa K2448011"
Line 3: | Line 3: | ||
<partinfo>BBa_K2448011 short</partinfo> | <partinfo>BBa_K2448011 short</partinfo> | ||
− | + | This part is the promoter region (0.36 kb upstream) of the PsiR gene of ''Agrobacterium tumefaciens'' str. C58 (Atu4743) | |
+ | |||
+ | pPsiR is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448006|BBa_K2448006]]) which is inhibited in the presence of D-Psicose [1]. | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | Our team used this pPsiR promoter together with the ''Agrobacterium tumefaciens'' PsiR transcription factor ([[Part:BBa_K2448006|BBa_K2448006]]) to build a Psicose biosensor ([[Part:BBa_K2448026|BBa_K2448026]]). The results show that pPsiR is functional in ''E. coli'' and that it behaved as predicted under and without induction. | ||
+ | |||
+ | Using the BPROM webserver [2], the predicted features of this promoter are: | ||
+ | Threshold for promoters - 0.20 | ||
+ | Promoter Pos: 299 LDF- 3.13 | ||
+ | -10 box at pos. 284 tggtgcaat Score 40 | ||
+ | -35 box at pos. 264 ttgcca Score 61 | ||
+ | Oligonucleotides from known TF binding sites: | ||
+ | rpoD17: TGCAATAA at position 287 Score - 5 | ||
<!-- --> | <!-- --> |
Revision as of 20:56, 28 October 2017
pPsiR from Agrobacterium tumefaciens
This part is the promoter region (0.36 kb upstream) of the PsiR gene of Agrobacterium tumefaciens str. C58 (Atu4743)
pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448006) which is inhibited in the presence of D-Psicose [1].
Usage and Biology
Our team used this pPsiR promoter together with the Agrobacterium tumefaciens PsiR transcription factor (BBa_K2448006) to build a Psicose biosensor (BBa_K2448026). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction.
Using the BPROM webserver [2], the predicted features of this promoter are: Threshold for promoters - 0.20 Promoter Pos: 299 LDF- 3.13 -10 box at pos. 284 tggtgcaat Score 40 -35 box at pos. 264 ttgcca Score 61 Oligonucleotides from known TF binding sites: rpoD17: TGCAATAA at position 287 Score - 5
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]