Difference between revisions of "Part:BBa K2271105"

 
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<partinfo>BBa_K2271105 short</partinfo>
 
<partinfo>BBa_K2271105 short</partinfo>
  
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<h1>PEX5</h1>
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<h2>Brief introduction</h2>
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<p>
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PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (<b>PTS1</b>), then folds itself to a more compact version and interacts with a variety of enzymes.
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<br>
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The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling.
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</p>
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<h2>Variant</h2>
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<p>
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We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism &minus; our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5.
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:28, 28 October 2017


PEX5 variant R15

PEX5

Brief introduction

PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (PTS1), then folds itself to a more compact version and interacts with a variety of enzymes.
The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling.

Variant

We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism − our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]