Difference between revisions of "Part:BBa K2455000:Experience"
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===Applications of BBa_K2455000=== | ===Applications of BBa_K2455000=== | ||
[[file:YNB_E_coli_growth.png|center]] | [[file:YNB_E_coli_growth.png|center]] | ||
| − | Growth of <i>E. coli</i> BL21 cells in Yeast Nitrogen Base medium lacking tryptophan. The strains used carried | + | Growth of <i>E. coli</i> BL21 cells in Yeast Nitrogen Base medium lacking tryptophan. The strains used carried biobricks involved in tryptophan synthesis and/or export under control of an IPTG-inducible T7 promoter. 1.0 mM IPTG was added after 3 hours of growth to induce expression. |
[[part:BBa_K2455004|YddG]]: Tryptophan, Tyrosine, Phenylalanine exporter. | [[part:BBa_K2455004|YddG]]: Tryptophan, Tyrosine, Phenylalanine exporter. | ||
Revision as of 19:54, 28 October 2017
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Applications of BBa_K2455000
Growth of E. coli BL21 cells in Yeast Nitrogen Base medium lacking tryptophan. The strains used carried biobricks involved in tryptophan synthesis and/or export under control of an IPTG-inducible T7 promoter. 1.0 mM IPTG was added after 3 hours of growth to induce expression.
YddG: Tryptophan, Tyrosine, Phenylalanine exporter.
AroG: Gene involved in Tryptophan, Tyrosine, and Phenylalanine synthesis.
TrpE: Gene involved in Tryptophan synthesis.
YddG-AroG: Double gene construct carrying the YddG and AroG genes.
YddG-TrpE: Double gene construct carrying the YddG and TrpE genes.
Control: Empty vector
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