Difference between revisions of "Part:BBa K2486004"
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<partinfo>BBa_K2486004 short</partinfo>
 
<partinfo>BBa_K2486004 short</partinfo>
  
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A part that constitutively generates the DtxR repressor.
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===Usage and Biology===
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BBa_J23108 BBa_B0034 BBa_K2486024 BBa_K2486005 BBa_K2486025 BBa_K2486006
 
The BBa_K486004 is a dtxR constitutive generator. This part is composed of:
 
The BBa_K486004 is a dtxR constitutive generator. This part is composed of:
  
 
# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection].
 
# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection].
 
# A ribosome binding site ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]).
 
# A ribosome binding site ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]).
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# A short spacer of 6bp (gtccta).
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# A short spacer of 6bp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2486024 BBa_K2486024]).
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# dtxR gene ([https://parts.igem.org/Part:BBa_K2486005 BBa K2486005]).
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# ''dtxR'' gene ([https://parts.igem.org/Part:BBa_K2486005 BBa_K2486005]).
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# A short spacer of 16bp (ccagctcagtccgcat).
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# A short spacer of 16bp ([https://parts.igem.org/Part:BBa_K2486024 BBa_K2486024]).
 
# A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
 
# A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
  
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We built this part in order to constitutively inhibit our synthetic promoter, the P<sub>dtxR</sub>
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We built this part in order to constitutively inhibit our synthetic promoter, the P<sub>dtxR</sub> wich is best described in parts BBa_K2486023, BBa_K2486014 and BBa_K2486009.
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REFERENCES:
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Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.
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De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.
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https://www.wikigenes.org/e/gene/e/2649688.html
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2486005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2486005 SequenceAndFeatures</partinfo>

Revision as of 19:16, 28 October 2017


dtxR generator

A part that constitutively generates the DtxR repressor.

Usage and Biology

BBa_J23108 BBa_B0034 BBa_K2486024 BBa_K2486005 BBa_K2486025 BBa_K2486006 The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection.
  2. A ribosome binding site (BBa_B0034).
  3. A short spacer of 6bp (BBa_K2486024).
  4. dtxR gene (BBa_K2486005).
  5. A short spacer of 16bp (BBa_K2486024).
  6. A synthetic terminator (BBa_K2486006).

We built this part in order to constitutively inhibit our synthetic promoter, the PdtxR wich is best described in parts BBa_K2486023, BBa_K2486014 and BBa_K2486009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]