Difference between revisions of "Part:BBa K2448007"
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<partinfo>BBa_K2448007 short</partinfo>
 
<partinfo>BBa_K2448007 short</partinfo>
  
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This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).
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This part is an E. coli codon optimized version of the PsiR found in ''Sinorhizobium fredii'' (NGR_b11520).
  
 
===Usage and Biology===
 
===Usage and Biology===
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PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
 
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
  
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Our team used this transcription factor together with the Sinorhizobium fredii PsiA or PsiR promoters ([[Part:BBa_K2448012|BBa_K2448012]], [[Part:BBa_K2448013|BBa_K2448013]]) to build two Psicose biosensors ([[Part:BBa_K2448028|BBa_K2448028]] and [[Part:BBa_K2448029|BBa_K2448029]]). The results shows that PsiR behaved as predicted under and without induction.
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Our team used this transcription factor together with the ''Sinorhizobium fredii'' pPsiA or pPsiR promoters ([[Part:BBa_K2448012|BBa_K2448012]], [[Part:BBa_K2448013|BBa_K2448013]]) to build two Psicose biosensors ([[Part:BBa_K2448028|BBa_K2448028]] and [[Part:BBa_K2448029|BBa_K2448029]]). The results show that PsiR behaved as predicted under and without induction.
  
 
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Revision as of 19:11, 28 October 2017


PsiR from Sinorhizobium fredii

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor together with the Sinorhizobium fredii pPsiA or pPsiR promoters (BBa_K2448012, BBa_K2448013) to build two Psicose biosensors (BBa_K2448028 and BBa_K2448029). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1024
  • 1000
    COMPATIBLE WITH RFC[1000]