Difference between revisions of "Part:BBa K2455007:Design"
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+ | Gu, P. <i>et al.</i> (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. <i>Microbial Cell Factories</i>, <b>11(1)</b>, p.30. | ||
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+ | Wang, J. <i>et al.</i> (2013). Genetic engineering of <i>Escherichia coli</i> to enhance production of l-tryptophan. <i>Applied Microbiology and Biotechnology</i>, <b>97(17)</b>, pp.7587-7596. |
Revision as of 18:42, 28 October 2017
YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1876
Illegal BglII site found at 1937 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 577
Illegal AgeI site found at 639
Illegal AgeI site found at 756 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
!
Source
The YddG gene is a synthetic codon optimized version of the E. coli MG1655 YddG gene.
The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.
References
Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.
Wang, J. et al. (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology, 97(17), pp.7587-7596.