Difference between revisions of "Part:BBa K2455007:Design"
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===Source=== | ===Source=== | ||
− | + | The YddG gene is a synthetic codon optimized version of the <i>E. coli</i> MG1655 YddG gene. | |
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+ | The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan ([https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-30 Gu <i>et al.</i>, 2012]), in addition a hexa his-tag has been added. | ||
===References=== | ===References=== |
Revision as of 18:41, 28 October 2017
YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1876
Illegal BglII site found at 1937 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 577
Illegal AgeI site found at 639
Illegal AgeI site found at 756 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
!
Source
The YddG gene is a synthetic codon optimized version of the E. coli MG1655 YddG gene.
The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.