Difference between revisions of "Part:pSB6A1"
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[[Image:MapI739201.jpg|left|thumb|'''Fig. 1:''' Map of the pBR322BB1 plasmid|250px]][[Image:Mappbr322.jpg|right|thumb|'''Fig. 2:''' Map of the original pBR322 plasmid|250px]] | [[Image:MapI739201.jpg|left|thumb|'''Fig. 1:''' Map of the pBR322BB1 plasmid|250px]][[Image:Mappbr322.jpg|right|thumb|'''Fig. 2:''' Map of the original pBR322 plasmid|250px]] | ||
| − | In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pBR322 was digested at its EcoRI and BamHI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original tetracyclin resistance and only ampicillin resistance is left. Since the original plasmid also contains a PstI restriction site in the bla gene, the GCA codon at this position was changed to GTA by site directed mutagenesis. The term "BB1" in | + | In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pBR322 was digested at its EcoRI and BamHI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original tetracyclin resistance and only ampicillin resistance is left. Since the original plasmid also contains a PstI restriction site in the bla gene, the GCA codon at this position was changed to GTA by site directed mutagenesis. The term "BB1" in pBR322BB1 refers to "BioBrick version 1". |
| − | + | <br> | |
===Purpose=== | ===Purpose=== | ||
| − | <p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part: | + | <p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739001 ''Part 1''], [https://parts.igem.org/wiki/index.php/Part:I739002 ''Part 2''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739003 ''Part 3''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739012 BBa_I739012] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739013 BBa_I739013]. More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here]. |
</p><br> | </p><br> | ||
Revision as of 08:22, 25 October 2007
No part name specified with partinfo tag.
Plasmid pBR322BB1 (Fig. 1) is based on pBR322 (Fig. 2), a low-copy cloning vector (15-20 copies per cell) that confers ampicillin (ApR) and tetracylin (TcR) resistance and harbours the pMB1 origin of replication.
In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pBR322 was digested at its EcoRI and BamHI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original tetracyclin resistance and only ampicillin resistance is left. Since the original plasmid also contains a PstI restriction site in the bla gene, the GCA codon at this position was changed to GTA by site directed mutagenesis. The term "BB1" in pBR322BB1 refers to "BioBrick version 1".
Purpose
This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 1, Part 2, Part 3 and the composites BBa_I739012 and BBa_I739013. More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here].
Sequence and Features No part name specified with partinfo tag.