Difference between revisions of "Part:BBa K2448008"

 
Line 3: Line 3:
 
<partinfo>BBa_K2448008 short</partinfo>
 
<partinfo>BBa_K2448008 short</partinfo>
  
−
PsiR Sm
+
This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium meliloti (SMb20483).
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
 +
 +
Our team used this transcription factor in some of our Psicose biosensors ([[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]) and the results shows that PsiR behaved as predicted under and without induction.
  
 
<!-- -->
 
<!-- -->

Revision as of 15:57, 28 October 2017


PsiR from Sinorhizobium meliloti

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium meliloti (SMb20483).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor in some of our Psicose biosensors (BBa_K2448030 and BBa_K2448031) and the results shows that PsiR behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1024
  • 1000
    COMPATIBLE WITH RFC[1000]