Difference between revisions of "Part:BBa K2448006"
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<partinfo>BBa_K2448006 short</partinfo> | <partinfo>BBa_K2448006 short</partinfo> | ||
| − | PsiR | + | This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site. | ||
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| + | Our team used this transcription factor in some of our Psicose biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]] and [[Part:BBa_K2448027|BBa_K2448027]]) and results shows that PsiR behaved as predicted under and without induction. | ||
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Revision as of 15:40, 28 October 2017
PsiR from Agrobacterium tumefaciens
This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4
Illegal AgeI site found at 691 - 1000COMPATIBLE WITH RFC[1000]