Difference between revisions of "Part:BBa K2271105:Design"
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This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it. | This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it. | ||
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https://static.igem.org/mediawiki/2017/c/c5/Artico_p5wt_polar.png | https://static.igem.org/mediawiki/2017/c/c5/Artico_p5wt_polar.png | ||
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===Source=== | ===Source=== | ||
Revision as of 14:24, 28 October 2017
PEX5 variant R15
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 409
- 1000COMPATIBLE WITH RFC[1000]
Part design
This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it. We thereby provide a construct which allows full control over the peroxisomes protein contents.
Source
Synthesized by IDT.