Difference between revisions of "Part:BBa K2271105:Design"
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This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it.
 
This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it.
 
We thereby provide a construct which allows full control over the peroxisomes protein contents.
 
We thereby provide a construct which allows full control over the peroxisomes protein contents.
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https://static.igem.org/mediawiki/2017/c/c5/Artico_p5wt_polar.png
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Revision as of 14:23, 28 October 2017


PEX5 variant R15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it. We thereby provide a construct which allows full control over the peroxisomes protein contents.

Artico_p5wt_polar.png



Source

Synthesized by IDT.

References