Difference between revisions of "Part:BBa K2448001"
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<partinfo>BBa_K2448001 short</partinfo>
 
<partinfo>BBa_K2448001 short</partinfo>
  
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Continuous engineering of the Green Fluorescent Protein from Aequorea victoria has led to several variants with improved features. Among the best is mEmerald for its photostability and resistance to bleaching. The protein contains the S65T and F64L mutations of eGFP and four others to improve maturation and folding at 37°C and increase the intrinsic brightness (20% more than eGFP).
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mEmerald is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C. The protein contains six mutations compared to wild-type: F64L; S65T; S72A; N149K; M153T; I167T; A206K [1].
  
 
This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
 
This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.

Revision as of 14:21, 28 October 2017


mEmerald

mEmerald is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C. The protein contains six mutations compared to wild-type: F64L; S65T; S72A; N149K; M153T; I167T; A206K [1].

This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.

Usage and Biology

Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.

Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]