Difference between revisions of "Part:BBa K2455001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In addition to having a M293T mutation the TrpE sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting. | + | In addition to having a M293T mutation the TrpE sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting. |
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===Source=== | ===Source=== | ||
Latest revision as of 14:05, 28 October 2017
His-tagged TrpE gene from E. coli MG1655
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 965
Illegal BglII site found at 1026 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In addition to having a M293T mutation the TrpE sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting.
Source
The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.
References
Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.