Difference between revisions of "Part:BBa K2448004"

Revision as of 12:45, 28 October 2017

mCherry

This codon optimized mCherry for Escherichia coli K12 comes from the biobrick (BBa_J06504). The optimization has been performed with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024)

Usage and Biology

mCherry is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching. Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques. Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024) and test our Psicose Biosensors (BBa_K2448025, BBa_K2448026, BBa_K2448027, BBa_K2448028, BBa_K2448029, BBa_K2448030 and BBa_K2448031) as well as our Fructose Biosensor (BBa_K2448032, ).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 570
Illegal AgeI site found at 682
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448004 short</partinfo>
-mCherry
-<!-- Add more about the biology of this part here
+This codon optimized mCherry for Escherichia coli K12 comes from the biobrick ([[Part:BBa_J06504|BBa_J06504]]). The optimization has been performed with help of JCAT ([http://www.jcat.de/]).
+Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]])
 ===Usage and Biology===
+mCherry is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching. Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
+Our team used this codon optimized mCherry to build the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]) and test our Psicose Biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]], [[Part:BBa_K2448027|BBa_K2448027]], [[Part:BBa_K2448028|BBa_K2448028]], [[Part:BBa_K2448029|BBa_K2448029]], [[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]) as well as our Fructose Biosensor ([[Part:BBa_K2448032|BBa_K2448032]], ).
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