Difference between revisions of "Part:BBa K2235010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The following primers were used to amplify the gblock: | |
| + | Fwd: CTAGTCTAGAATGTTTGTCTTTATGTTACTATTGCTGCTACCGTTTA | ||
| + | Rev: AAAACTGCAGTTATTCTACAGGAATAAGTTGCCATTGACTTGTCCAATAAGTATTTGGAA | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:43, 28 October 2017
Endo beta galactosidase with T7 promoter, RBS and functional unit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 246
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The following primers were used to amplify the gblock:
Fwd: CTAGTCTAGAATGTTTGTCTTTATGTTACTATTGCTGCTACCGTTTA
Rev: AAAACTGCAGTTATTCTACAGGAATAAGTTGCCATTGACTTGTCCAATAAGTATTTGGAA
Source
Endo beta galactosidase with T7 promoter, RBS and functional unit