Difference between revisions of "Part:BBa K2448016"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2448016 short</partinfo> | <partinfo>BBa_K2448016 short</partinfo> | ||
| − | This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter (BBa_K864400) [1] and the consensus binding site of PsiR regulator of Rhizobiale [2]. | + | This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]) [1] and the consensus binding site of PsiR regulator of Rhizobiale [2]. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI (BBa_K864400), regulated by LacI to make it psicose inducible. | + | D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI ([[Part:BBa_K864400|BBa_K864400]]), regulated by LacI to make it psicose inducible. |
| − | pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006). PsiR is a LacI family transcription factor interacting with psicose, this is why the regulation of its promoter is similar. | + | pPsiTac1 is a synthetic promoter derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR ([[Part:BBa_K2448006|BBa_K2448006]]). PsiR is a LacI family transcription factor interacting with psicose, this is why the regulation of its promoter is similar. |
| − | As shown by the characterization of our psicose biosensor (BBa_K2448027), pPsiTac1 is strongly regulated by PsiR thanks to the consensus sequence, and the reporter gene shows high level expression under psicose induction. pPsiTac1 can therefore be categorized as a strong psicose inducible promoter. | + | As shown by the characterization of our psicose biosensor ([[Part:BBa_K2448027|BBa_K2448027]]), pPsiTac1 is strongly regulated by PsiR thanks to the consensus sequence, and the reporter gene shows high level expression under psicose induction. pPsiTac1 can therefore be categorized as a strong psicose inducible promoter. |
<!-- --> | <!-- --> | ||
Revision as of 11:56, 28 October 2017
pPsiTac1
This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter (BBa_K864400) [1] and the consensus binding site of PsiR regulator of Rhizobiale [2].
Usage and Biology
D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI (BBa_K864400), regulated by LacI to make it psicose inducible.
pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006). PsiR is a LacI family transcription factor interacting with psicose, this is why the regulation of its promoter is similar.
As shown by the characterization of our psicose biosensor (BBa_K2448027), pPsiTac1 is strongly regulated by PsiR thanks to the consensus sequence, and the reporter gene shows high level expression under psicose induction. pPsiTac1 can therefore be categorized as a strong psicose inducible promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]