Difference between revisions of "Part:BBa K2455001:Design"

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===References===
 
===References===
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Gu, P. <i>et al.</i> (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. <i>Microbial Cell Factories</i>, <b>11(1)</b>, p.30.

Revision as of 11:23, 28 October 2017


His-tagged TrpE gene from E. coli MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 965
    Illegal BglII site found at 1026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In addition to having a M293T mutation the TrpE sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting.


Source

The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.

References

Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.