Difference between revisions of "Part:BBa K2235008"

Revision as of 11:20, 28 October 2017

Endo beta galactosidase

Usage and Biology

The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates fro=m the species Clostridium Perfringens.

Figure 1: Schematic representation of EBG enzyme reaction mechanism.

You can find more details about endo-beta galactosidase in its composite part BBa_K2235010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 174
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates fro=m the species Clostridium Perfringens.
+[[File:EBGComp_Mechanism.png|600px|thumb|left|Figure 1: Schematic representation of EBG enzyme reaction mechanism.]]
-Figure x: mechanism of endo-beta galactosidase enzyme
 You can find more details about endo-beta galactosidase in its composite part BBa_K2235010.