Difference between revisions of "Part:BBa K2455001"
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TrpE codes for the Anthranilate enzyme responsible for the first step of the conversion of Chorismate into to tryptophan. | TrpE codes for the Anthranilate enzyme responsible for the first step of the conversion of Chorismate into to tryptophan. | ||
| − | Previous work ( | + | Previous work ([https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-30 Gu <i>et al.</i>, 2012]) have shown that overexpression of TrpE leads to increased tryptophan production in E. coli. |
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Latest revision as of 11:18, 28 October 2017
His-tagged TrpE gene from E. coli MG1655
TrpE gene from E. coli strain MG1655 carrying an M293T point mutation (to avoid negative feedback inhibition) and a hexa histidine-tag attached by a short linker sequence.
TrpE codes for the Anthranilate enzyme responsible for the first step of the conversion of Chorismate into to tryptophan.
Previous work (Gu et al., 2012) have shown that overexpression of TrpE leads to increased tryptophan production in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 965
Illegal BglII site found at 1026 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]